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The second response cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as items (Fig. 9) - UV/Vis. As drawn in Fig - https://www.livebinders.com/b/3570027?tabid=514355ed-03f4-acee-f8e7-d79f6b7bffab. 9, the reaction might also include electron transfer from H4B to the Fe, IIO2 intermediate


l9 As displayed in Fig. 9, this could be since the H4B extreme acts as an electron sink in a later point in the reaction, so as to enable NOS UV/Vis/NIR to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.